Mevinolin: a highly specific inhibitor of microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase of radish plants.
نویسندگان
چکیده
The fungal metabolite mevinolin, known to be a potent hypocholesterolemic agent, exerts in vitro a strong inhibitory effect on microsomal HMG-CoA reductase from etiolated radish seedlings at a concentration of about three magnitudes lower than the Km towards the natural substrate (S)-HMG-CoA (I50 = 2.5 x 10(-9)M). Beside this, mevinolin significantly inhibits the root elongation of radish as well as of wheat seedlings already at low concentrations of 10 to 100 ppb (= 2.5 x 10(-8) to 2.5 x 10(-7)M).
منابع مشابه
Mechanisms of inhibition by mevinolin (MK 803) of microsome-bound radish and of partially purified yeast HMG-CoA reductase (EC.1.1.1.34).
1) In kinetic studies, mevinolin proved to be a highly specific inhibitor of partially purified yeast HMG-CoA reductase (Ki = 3.5 nM towards HMG-CoA) and of microsomal HMG-CoA reductase from etiolated radish seedlings (Ki = 2.2 nM). At low concentrations of NADPH, the inhibitor counteracts the sigmoidal response of plant HMG-CoA reductase activity towards the cosubstrate. At higher concentratio...
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ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. Section C, Biosciences
دوره 37 1-2 شماره
صفحات -
تاریخ انتشار 1982